| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089539 |
| Chromosome: | chromosome 1 |
| Location: | 2827782 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016900 | (1 of 1) PF14347 - Domain of unknown function (DUF4399) (DUF4399) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCACAACGGACTGGCGGCCACGCTGTGG |
| Internal bar code: | GTGACACGGTAATCAGGATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 99.2018 |
| LEAP-Seq n confirming: | 7208 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGTGAAGCTATTTGTGC |
| Suggested primer 2: | GGGACTGGTGCTGCTAATGT |