| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089547 |
| Chromosome: | chromosome 9 |
| Location: | 6705693 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408851 | (1 of 2) 3.4.11.9 - Xaa-Pro aminopeptidase / X-Pro aminopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTAGACGAGGGGGGGGATTACATTCAT |
| Internal bar code: | AATTTTTGTGAGATTACAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 99.5825 |
| LEAP-Seq n confirming: | 9780 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCACACACACACACACA |
| Suggested primer 2: | AAACAACAAAACAGGCCCAG |