| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089547 |
| Chromosome: | chromosome 9 |
| Location: | 6705733 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408851 | (1 of 2) 3.4.11.9 - Xaa-Pro aminopeptidase / X-Pro aminopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTCTACCACTTTCTTAACTAGTCACG |
| Internal bar code: | TAGTTAGTGGTATCTGAGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 28 |
| LEAP-Seq percent confirming: | 99.5413 |
| LEAP-Seq n confirming: | 868 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAACAAAACAGGCCCAG |
| Suggested primer 2: | GAGGCACACACACACACACA |