| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089552 |
| Chromosome: | chromosome 1 |
| Location: | 3252167 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020400 | (1 of 1) K13111 - WD40 repeat-containing protein SMU1 (SMU1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACCGTAGCCACCACCCACGCCCCAGTCA |
| Internal bar code: | CAGCTGGGGGGCGAGTACGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 99.7231 |
| LEAP-Seq n confirming: | 5402 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCTCTCACCTGCCCTTC |
| Suggested primer 2: | GGTTGCTCAGTGTAGCGTGA |