Insertion junction: LMJ.RY0402.089572_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.089572
Chromosome:chromosome 16
Location:5897097
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g677200 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTGCGGGTCGCAATCCTTCCACGCCTCT
Internal bar code:AAGACGGGGTCCAACAGAGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:455
LEAP-Seq percent confirming:35.352
LEAP-Seq n confirming:467
LEAP-Seq n nonconfirming:854
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TAGATCCAGGTATCGCCCAG
Suggested primer 2:CTTCGTCGCTTAAACTTGCC