| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089572 |
| Chromosome: | chromosome 16 |
| Location: | 5897099 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677200 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGCGGTGCGGGACCTGCGGTCCTCGC |
| Internal bar code: | CGGGTTACGCTTCGTCTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 864 |
| LEAP-Seq percent confirming: | 98.7155 |
| LEAP-Seq n confirming: | 2536 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGATCCAGGTATCGCCCAG |
| Suggested primer 2: | CTTCGTCGCTTAAACTTGCC |