| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089592 |
| Chromosome: | chromosome_6 |
| Location: | 1163065 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257250 | BBS2 | Bardet-Biedl syndrome-2 associated protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGATCAAACGGCGGTTGGAGCCGTCCATG |
| Internal bar code: | GTTCGTCAAATAAGCGTGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 93.274 |
| LEAP-Seq n confirming: | 5242 |
| LEAP-Seq n nonconfirming: | 378 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACATGTGGACGGCATAGTG |
| Suggested primer 2: | ATGAATGCTTCAGGAATCGG |