| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089599 |
| Chromosome: | chromosome 16 |
| Location: | 3615678 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684505 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCTTGCACGCCCCCGCACCCGTTGCC |
| Internal bar code: | GTCTGGTTCTGAGCCCATGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 144 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGAAACAGCTCCAAGG |
| Suggested primer 2: | CAGATCTACCTCCTCCACCG |