| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089617 |
| Chromosome: | chromosome 6 |
| Location: | 7015799 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g296800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACCGGTAATGGAATTGCGCCGCACACTG |
| Internal bar code: | TTAGGCGAAGTGAGATGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 114 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACCCGTCATGCCTGTAGT |
| Suggested primer 2: | ACAACTCGCAACCAAGGAAC |