Insertion junction: LMJ.RY0402.089617_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089617
Chromosome:chromosome 6
Location:7016159
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g296850 FAP81,DLEC1 (1 of 8) IPR008962 - PapD-like; Flagellar Associated Protein 81 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACCAACGACAGCAGCCAAAGCACGGTCC
Internal bar code:GGACCCTGGCGGAAGGTCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:310
LEAP-Seq percent confirming:60.1695
LEAP-Seq n confirming:426
LEAP-Seq n nonconfirming:282
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCGTCATGCCTGTAGT
Suggested primer 2:GCATAGAGGAGACGGACGAG