| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089636 |
| Chromosome: | chromosome 6 |
| Location: | 602323 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253250 | MADS2 | (1 of 1) PTHR11945:SF221 - SERUM RESPONSE FACTOR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCATTGGCATGGACGCCACGAAGCAC |
| Internal bar code: | TCCTTGCAGGGTCCAGGTGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 94.8718 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAAAAATGGATGGGTGC |
| Suggested primer 2: | CTTACCTGAATGCGCTCTCC |