| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089656 |
| Chromosome: | chromosome 13 |
| Location: | 3666292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588850 | (1 of 3) PF10250 - GDP-fucose protein O-fucosyltransferase (O-FucT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCATGCGGTCTGCTTGCAGGACAAAGTCA |
| Internal bar code: | ATTGTGGGCTGCGGGTATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 240 |
| LEAP-Seq percent confirming: | 69.5582 |
| LEAP-Seq n confirming: | 3448 |
| LEAP-Seq n nonconfirming: | 1509 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACTTGGGGTGGACAGAGG |
| Suggested primer 2: | GGGACTCCCCTGACCTTTAG |