| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089664 |
| Chromosome: | chromosome 6 |
| Location: | 3500047 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCTTAACTCGCAGAGTCCCGCTATCATC |
| Internal bar code: | TAATGGTCCAATACCGTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.8949 |
| LEAP-Seq n confirming: | 1901 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCGGACTGCTAGCGAAC |
| Suggested primer 2: | ACACAAGGGTCGTGGCTTAC |