| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089665 |
| Chromosome: | chromosome 12 |
| Location: | 5606846 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532000 | (1 of 3) PF04359 - Protein of unknown function (DUF493) (DUF493) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGCCCTTGCAAACCGCTCCCTGCTTAC |
| Internal bar code: | CCCTGACGTACGCCCCGTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 52.3738 |
| LEAP-Seq n confirming: | 3839 |
| LEAP-Seq n nonconfirming: | 3491 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGGTTGCACCTGCTGA |
| Suggested primer 2: | GGTGTCCACGCTTTACCTGT |