| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089676 |
| Chromosome: | chromosome 8 |
| Location: | 4935020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385300 | HLM14 | Histone-lysine N-methyltransferase; (1 of 6) PTHR12197//PTHR12197:SF138 - SET AND MYND DOMAIN CONTAINING // SET AND MYND DOMAIN-CONTAINING PROTEIN DDB_G0292140 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTGGGGCCGGGAGGGGCAAAGGGTAG |
| Internal bar code: | TATATCCCGCCAGAAAAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 386 |
| LEAP-Seq percent confirming: | 99.7732 |
| LEAP-Seq n confirming: | 9679 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCGTTCTCCATGCTCAA |
| Suggested primer 2: | CGAGTACATCTCATAAGCGGC |