| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.089686 |
| Chromosome: | chromosome 7 |
| Location: | 3896641 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339100 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTACGGGTCTGCCTACCCCGGTGGTGGGTC |
| Internal bar code: | TAAGAGTCGGCGTTGCGAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 55.515 |
| LEAP-Seq n confirming: | 760 |
| LEAP-Seq n nonconfirming: | 609 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTAACCCTATGTGGGCT |
| Suggested primer 2: | AGTCCATCGGCTTTGTCATC |