| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089724 |
| Chromosome: | chromosome 10 |
| Location: | 2779327 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGGCACTCCTCCATCTCACGCTGCGCC |
| Internal bar code: | TCGAAGGCCGGAACACGCAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 86 |
| LEAP-Seq percent confirming: | 74.6441 |
| LEAP-Seq n confirming: | 839 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTCGTCGTCATCAACCT |
| Suggested primer 2: | CTCGGCTGTCTGAGGAGAAC |