| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089746 |
| Chromosome: | chromosome 17 |
| Location: | 5559325 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g737525 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGGTTGGTCAGCCTGGACGTCACGGC |
| Internal bar code: | CTGTGAATTGAGGTAGCAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 97.7239 |
| LEAP-Seq n confirming: | 3263 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACCTGCTTCGGTCAGTC |
| Suggested primer 2: | CTAGATGGCGCAGGGCTA |