| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089773 |
| Chromosome: | chromosome 4 |
| Location: | 3736841 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229350 | (1 of 1) 2.1.1.290 - tRNA(Phe) (7-(3-amino-3-carboxypropyl)wyosine(37)-O)-methyltransferase / tRNA-yW synthesizing enzyme-4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTAGGGCCACAGCCACAGCCGCCGCCAC |
| Internal bar code: | GATGGCTATCTCACATCGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 98.2955 |
| LEAP-Seq n confirming: | 346 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGTGGGAAAGGGTAGT |
| Suggested primer 2: | CTGCGTTAAGCCCCATACAT |