| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089805 |
| Chromosome: | chromosome 12 |
| Location: | 8898844 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544450 | CSE20 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGTTGAGGAAGGGGGCACCCTCCAGGCCA |
| Internal bar code: | TGCCGTCCGGCGAGGGTCGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 97.9541 |
| LEAP-Seq n confirming: | 1963 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGAGCTTGGTAAACGAGG |
| Suggested primer 2: | GCCAAGCTGAACTTCCAGAC |