| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089807 |
| Chromosome: | chromosome 2 |
| Location: | 8453385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141706 | ERA1 | Putative chloroplast 16S rRNA processing factor; (1 of 1) K03595 - GTP-binding protein Era (era) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCACATGCCATATCTTGACTTGTCTTGA |
| Internal bar code: | AGGTGCCGTTAGGGGCGGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 1240 |
| LEAP-Seq n nonconfirming: | 372 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |