Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.089814 |
Chromosome: | chromosome_14 |
Location: | 2462315 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g624950 | DHC10,IDA2,DYH1B | Flagellar inner arm dynein 1 heavy chain beta | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CACCCGCACCCACAGCTCGTTCGTGATCCC |
Internal bar code: | AATCAGCGGGTGGATGTAACCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 303 |
LEAP-Seq percent confirming: | 98.7484 |
LEAP-Seq n confirming: | 789 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AATTTCACAGGCCATCAAGG |
Suggested primer 2: | TACAGCTCGTCGTTGGACAG |