| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089824 |
| Chromosome: | chromosome 13 |
| Location: | 3371891 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586800 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGACCAGCCGCTTCAGCTTGTCCGCCT |
| Internal bar code: | ATCTAGCAGACCCGTTGCTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 99.6151 |
| LEAP-Seq n confirming: | 2329 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACTGGCGTGCTGATGT |
| Suggested primer 2: | TCATAGGAGGAAACCAACGC |