| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089834 |
| Chromosome: | chromosome 2 |
| Location: | 4910095 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103550 | EIF1A | Eukaryotic translation initiation factor 1A, eIF-1A; (1 of 1) K03236 - translation initiation factor 1A (EIF1A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGATGACGTGGGCGGAGGGCATGAGGT |
| Internal bar code: | AAGCGATGATGATACGGGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGGGCTGAATAGCAAGC |
| Suggested primer 2: | CGAGCTGGTCTACAAGGAGG |