| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089855 |
| Chromosome: | chromosome 1 |
| Location: | 5741748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACCACGCGCGGGCGCGGACTGCTGCAG |
| Internal bar code: | GACTCATATAATTATTTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 227 |
| LEAP-Seq percent confirming: | 92.144 |
| LEAP-Seq n confirming: | 8879 |
| LEAP-Seq n nonconfirming: | 757 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCAGTGTCGCTTATCAA |
| Suggested primer 2: | CGATGTGTCTCAAAGGCTCA |