| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089870 |
| Chromosome: | chromosome 4 |
| Location: | 2393594 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221150 | GT90-5,GT90F5 | GT90 family protein 5; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGTGCCTCCCTTCCCTCCTCTCCCACG |
| Internal bar code: | GGTCAGGGTGTGCTGTGTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.398 |
| LEAP-Seq n confirming: | 1651 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTGGTGGCAGGTGAATCT |
| Suggested primer 2: | GTGCATAGTTTGGGGCAGTT |