Insertion junction: LMJ.RY0402.089874_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.089874
Chromosome:chromosome 14
Location:1990770
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g621400 (1 of 1) IPR001810//IPR025886 - F-box domain // Phloem protein 2-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGCACGCACACGAGACCCACTCGAGGCC
Internal bar code:CAGCGAGCGCTGAAATGTCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:82.4837
LEAP-Seq n confirming:631
LEAP-Seq n nonconfirming:134
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TAGACAGATCTGGGACGCCT
Suggested primer 2:AAATGGCGAAATCAGGACAC