| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089890 |
| Chromosome: | chromosome 3 |
| Location: | 3428732 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167150 | FMO1 | Flavin-containing monooxygenase; (1 of 2) PTHR23023//PTHR23023:SF4 - DIMETHYLANILINE MONOOXYGENASE // FLAVIN-CONTAINING MONOOXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAAAGTCCCGGTCTGGGTGTCGCAAGTC |
| Internal bar code: | ACTCGGTTATGGCTCTGCATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 715 |
| LEAP-Seq percent confirming: | 99.4681 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAAAGGTCCACGTTGCG |
| Suggested primer 2: | GCTAGCACAATCGGAGAAGG |