Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.089890 |
Chromosome: | chromosome_9 |
Location: | 3443131 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g389282 | FAP114 | Flagellar Associated Protein, C1a-32 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGATGATTGTGTGGTTTGTGTACAGTAAT |
Internal bar code: | TCTGTCGGGCCATGACCGCCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 134 |
LEAP-Seq percent confirming: | 42.9773 |
LEAP-Seq n confirming: | 511 |
LEAP-Seq n nonconfirming: | 678 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTTATACCCAGTCCCTGCCA |
Suggested primer 2: | GTTGAACCGCATCCTGATCT |