| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.089898 |
| Chromosome: | chromosome 8 |
| Location: | 2336435 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370650 | PGL2 | (1 of 2) 3.1.1.31 - 6-phosphogluconolactonase; 6-phosphogluconolactonase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGGCGTGCCGGGCGCGTCCATGCCCGT |
| Internal bar code: | ATTGGGGTTTTCTCTATTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 222 |
| LEAP-Seq percent confirming: | 99.6109 |
| LEAP-Seq n confirming: | 512 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTACGAGTCCAAGCTGC |
| Suggested primer 2: | CAAGTAGTCATTCAGGCGCA |