| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089920 |
| Chromosome: | chromosome_9 |
| Location: | 3095235 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g386850 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGCACGACCCACATTCGGACTTCACGACGG |
| Internal bar code: | CAGTCCCGCGACGCGGTGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 41 |
| LEAP-Seq percent confirming: | 99.3168 |
| LEAP-Seq n confirming: | 1163 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCACACAACCGCACACC |
| Suggested primer 2: | TGACCTCCAAAATCCTCTGC |