| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089926 |
| Chromosome: | chromosome_11 |
| Location: | 2544232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g476050 | ODA2,DHC15,DYHG | Flagellar outer arm dynein heavy chain gamma | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTAGGAGGCGCGCAGGCCGGCCTTGATG |
| Internal bar code: | CTGGCGGGCCTATCCTGACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 95.0392 |
| LEAP-Seq n confirming: | 728 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGCCCAAGGACTGGAT |
| Suggested primer 2: | ATGTTGAGGCGGTCAATCTC |