| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.089942 |
| Chromosome: | chromosome 7 |
| Location: | 1791578 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325723 | ADK1 | Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF85 - NUCLEOTIDE KINASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTCGCCCTCACCCTCCTCACCGCCCC |
| Internal bar code: | GACTCGTTTTTACATGGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 98.6438 |
| LEAP-Seq n confirming: | 5455 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTTTATGTGGAAGCGGT |
| Suggested primer 2: | GCAGAGAGGTCTCAACCCTG |