| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.089975 |
| Chromosome: | chromosome 2 |
| Location: | 2853731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094750 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTAGTTTCCTGCTGGCCTTCGCAGCCAAG |
| Internal bar code: | GAGTATTCTCGGGCTAGTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 98.8197 |
| LEAP-Seq n confirming: | 4270 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGTGGAGACGGAAGGAA |
| Suggested primer 2: | CAAATGTGTTCAGCCCAATG |