Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.089986 |
Chromosome: | chromosome 17 |
Location: | 1107745 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g704000 | (1 of 2) 1.1.3.30 - Polyvinyl-alcohol oxidase / PVA oxidase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCCCCAACCACTCATAGCGACTCACC |
Internal bar code: | AAGACACCGTGCCCGCGCGTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 570 |
LEAP-Seq percent confirming: | 98.5429 |
LEAP-Seq n confirming: | 4193 |
LEAP-Seq n nonconfirming: | 62 |
LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGTCCTACTGTTCCAGCA |
Suggested primer 2: | ATGTCAGCCCCTGATTATGC |