| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.090002 |
| Chromosome: | chromosome 12 |
| Location: | 4376424 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520400 | uL4m,MRPL4 | Mitochondrial ribosomal protein L4; (1 of 2) K02926 - large subunit ribosomal protein L4 (RP-L4, MRPL4, rplD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACTTCGTGCGTCCACCCAGCGCCGTGC |
| Internal bar code: | GGGTCGCCCTGTTGTTCATTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 694 |
| LEAP-Seq percent confirming: | 99.2021 |
| LEAP-Seq n confirming: | 373 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACGGAGTAGAGAGGATCG |
| Suggested primer 2: | GGGATCAGGCCTGTGATAGA |