| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090007 |
| Chromosome: | chromosome 16 |
| Location: | 7168230 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681238 | (1 of 1) K03650 - tRNA modification GTPase [EC:3.6.-.-] (mnmE, trmE, MSS1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTAGGATTTGGGACTAAAGATGTGGTGA |
| Internal bar code: | TGGGCGCCGACAACGAGCTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 73 |
| LEAP-Seq percent confirming: | 85.5346 |
| LEAP-Seq n confirming: | 1632 |
| LEAP-Seq n nonconfirming: | 276 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGGGAACTTACATTGGTG |
| Suggested primer 2: | CGGGAGATGTTTTGTCGAAT |