Insertion junction: LMJ.RY0402.090017_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.090017
Chromosome:chromosome 3
Location:2131463
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g157100 (1 of 1) PF03420 - Prohead core protein serine protease (Peptidase_S77) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCTGTAGTCGGCGATGCGTGCCTGCGGG
Internal bar code:CGCGACGCCGTGAGCGCTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:97.8077
LEAP-Seq n confirming:9146
LEAP-Seq n nonconfirming:205
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CGACATTGACAACGTGATCC
Suggested primer 2:CAGCATTCCACAAACACACC