| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090027 |
| Chromosome: | chromosome_13 |
| Location: | 1619020 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g573500 | FAP95 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGGCATCCAGGGGCACGGAATTGCCTAGT |
| Internal bar code: | TCCGGGGCTTCATGCGTGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 698 |
| LEAP-Seq percent confirming: | 99.5854 |
| LEAP-Seq n confirming: | 5044 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATGAGGGTGAGAGTG |
| Suggested primer 2: | TGTCTATGCTTGGCGTTGAG |