| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090027 |
| Chromosome: | chromosome_13 |
| Location: | 1619034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g573500 | FAP95 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACTGCCCTTCTCGCCAGCGGCGGCGGCC |
| Internal bar code: | CCACCCGCGGTCGACAGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 94.729 |
| LEAP-Seq n confirming: | 3828 |
| LEAP-Seq n nonconfirming: | 213 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATGAGGGTGAGAGTG |
| Suggested primer 2: | TGTCTATGCTTGGCGTTGAG |