| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090046 |
| Chromosome: | chromosome 9 |
| Location: | 2117391 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393650 | (1 of 1) PF00069//PF01590 - Protein kinase domain (Pkinase) // GAF domain (GAF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCCTAGCCCGACCCACCTCGCCAGA |
| Internal bar code: | GACGAGGGCGAAGCACTGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 541 |
| LEAP-Seq percent confirming: | 57.0776 |
| LEAP-Seq n confirming: | 375 |
| LEAP-Seq n nonconfirming: | 282 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCAGAACTCCATGATGA |
| Suggested primer 2: | GTGTACTTCGGCACCTGGAT |