| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.090076 |
| Chromosome: | chromosome 16 |
| Location: | 3072743 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g665300 | (1 of 1) PTHR24006:SF329 - UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 44 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGGCCATAGCGGGTACCCACACGCTGT |
| Internal bar code: | GAGTGTATGCCCGCACGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 716 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTCCAGGTTGGGTAG |
| Suggested primer 2: | ACGCTTAACCAGCTCAAGGA |