| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.090087 |
| Chromosome: | chromosome 16 |
| Location: | 958487 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g648750 | (1 of 1) PF10373 - Est1 DNA/RNA binding domain (EST1_DNA_bind) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCGTGCTGGCGCTGCTGGTCGGGTGCG |
| Internal bar code: | GAACGATTGCTGTCGTCGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 99.7266 |
| LEAP-Seq n confirming: | 8391 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACACATCTCCTCTTGCG |
| Suggested primer 2: | CTCGGTTTAGGCTGAGTTCG |