Insertion junction: LMJ.RY0402.090102_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.090102
Chromosome:chromosome 6
Location:1790899
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g262400 (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCACCTGCGCCACGCACACGCTGCTCCA
Internal bar code:GCCGAAGTTATTGGGTAGGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:309
LEAP-Seq percent confirming:1.81818
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:432
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAAGACCAGCAGGGACTC
Suggested primer 2:AAGCTCTTCTCCAACATCGC