Insertion junction: LMJ.RY0402.090107_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.090107
Chromosome:chromosome_2
Location:8407045
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g141606 DHC9 Flagellar inner arm dynein heavy chain, dynein c (monomeric) antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ACCTTCTTGATGTCCTCGTGCCACTCGGTC
Internal bar code:ACACCATCGGGGGGGGCGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:397
LEAP-Seq percent confirming:71.502
LEAP-Seq n confirming:1252
LEAP-Seq n nonconfirming:499
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGTATCAACAACCAACCGCC
Suggested primer 2:GAGTCTTCTACGACCGCCTG