Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.090141 |
Chromosome: | chromosome 2 |
Location: | 3655128 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g095151 | (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase | 5'UTR_intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACTGTGTGATGCAGCTAGGTCCAGGGA |
Internal bar code: | GGAGGCTTAGGTTCTCCTGTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 334 |
LEAP-Seq percent confirming: | 87.722 |
LEAP-Seq n confirming: | 1729 |
LEAP-Seq n nonconfirming: | 242 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCACCTGTTCGACGTATCCT |
Suggested primer 2: | GCCAACACACACATTCCAAG |