Insertion junction: LMJ.RY0402.090148_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.090148
Chromosome:chromosome 4
Location:155625
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g216875 (1 of 173) IPR013083 - Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCGGTTGCTCATTCTGCTTCGCGCTCA
Internal bar code:AACACCGGTCCTTTCGCACTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:329
LEAP-Seq percent confirming:54.5702
LEAP-Seq n confirming:2406
LEAP-Seq n nonconfirming:2003
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ATCTCCTCACTTGTCCGCTG
Suggested primer 2:GCTGCTGCTGTTGTTGTTGT