Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.090168 |
Chromosome: | chromosome 6 |
Location: | 1647373 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g260950 | (1 of 1) K12483 - EH domain-containing protein 1 (EHD1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCAAAACCCCACACACAACCCTCCCGC |
Internal bar code: | TGGCGTCTCTTCGGAACCTATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 79 |
LEAP-Seq percent confirming: | 99.3151 |
LEAP-Seq n confirming: | 145 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGACAAACACACTTCCCCTT |
Suggested primer 2: | TGCTGCACTACTGTTCCTGG |