Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.090171 |
Chromosome: | chromosome 8 |
Location: | 3718613 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre08.g376950 | MMP14 | Matrix metalloproteinase; (1 of 40) 3.4.24.38 - Gametolysin / Lysin | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGAGGGGTTCGCTGGCGCCCGGCGTGT |
Internal bar code: | TCAGAAGTCTCTTTAACCCCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 928 |
LEAP-Seq percent confirming: | 99.7063 |
LEAP-Seq n confirming: | 15953 |
LEAP-Seq n nonconfirming: | 47 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACTCTCTTCGCTGGTCGGT |
Suggested primer 2: | CAGCGGTACCAACAGCAGTA |