| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.090182 |
| Chromosome: | chromosome 1 |
| Location: | 4238545 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028400 | DDC1 | Aromatic-aminoacid decarboxylase; (1 of 102) PF01753 - MYND finger (zf-MYND) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACGCGGACGTGCAGATGAGGCCCTCGG |
| Internal bar code: | AATCGGTGTTGCGCTAAGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 33 |
| LEAP-Seq percent confirming: | 85.1064 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACAAGCTGGACTCGCA |
| Suggested primer 2: | GAATGATGAACAGAGCCGGT |